Process Management

News 286 views last update:7 Aug 2012

Neogen launches NeoColumn for T-2/HT-2 toxins

In response to imminent regulation in the European Union, Neogen has expanded its comprehensive line of mycotoxin detection products to include NeoColumn T-2/HT-2 for detecting Fusarium toxins.

NeoColumn T-2/HT-2 is an immunoaffinity column (IAC) for the detection of Fusarium spp. toxins T-2 and HT-2.
 
In addition to new IAC assay, Neogen Europe also developed a simple isocratic HPLC method for the detection of T-2/HT-2.
 
NeoColumn T-2/HT-2 is a highly effective IAC, which can recover T-2 and HT-2 from a broad range of matrices including horse feed, wheat and oats.
 
Pre-detection
The new assay is a high performance IAC designed for the easy clean-up and concentration of sample prior to analyses such as HPLC, GC-MS and ELISA.
 
A representative sample is extracted and filtered before being passed through the highly specific column.
 
Binding takes place between immobilised antibody and T-2/HT-2 toxin. The column is then washed to remove any unbound material.
 
Captured T-2/HT-2 is released by denaturing the antibody using a single methanol elution.
 
For HPLC assay, the eluted sample is firstly subjected to derivitisation with 4-dimethylaminopyridine (DMAP) and 1-anthroylnitrile (1-AN), then reconstituted in an appropriate solvent.
 
Related website: Neogen Europe
 
 
 

Dick Ziggers

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