News 434 views last update:6 Aug 2012

HPLC method for detecting arsenic in feed

A novel method has been developed to detect two organic arsenic animal feed additives including roxarsone and p-arsanilic acid, as well as other arsenic species, by using high-performance liquid chromatography coupled to an inductively coupled plasma mass spectrometer (HPLC-ICP-MS).

The influence of the type and concentrations of ion-pairing reagents on the separation efficiency of the different arsenic compounds was examined.
The effects of the mobile phase pH on the retention of arsenic species on the chromatography column were studied.
When a gradient elution procedure was used, the best separation of seven arsenic species could be achieved in <20 min.
Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used as an assistant tool to screen arsenobetain (AsB) in the feed samples.
The extractions of arsenic compounds from formula feed samples were studied, and results showed that the extraction with methanol/water (1:1) mixture yielded the most efficient percent compound recovery and the fastest extraction time for all arsenic species.
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Dick Ziggers

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